perform a first-hand investigation to:
- extract the mixture of pigments from leaves
- examine the absorption spectrum of these pigments
- separate the pigments using chromatography
How you can measure the amount of pigment in plants Mad Science Network, Washington University, USA
Be aware that more sophisticated chromatography techniques than using solvents are now available to separate pigments in plants. Advanced spectrophotometers can precisely determine the absorption spectrum of plant pigments.
Extraction of pigments from spinach Classroom bats, Bermuda Biological Station for Research, Inc, Florida, USA