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4.2 plan and perform a first-hand investigation to test the conditions that influence the rate of enzyme activity

plan and perform a first-hand investigation to test the conditions that influence the rate of enzyme activity

The effect of pH on catalase
Aim : To examine the rate of reaction of the enzyme catalase at different pH levels.

Method: Fill six identical test tubes with 20mL of 10% hydrogen peroxide solution. The pH of each test tube is then adjusted by adding drops of sulfuric acid or sodium hydroxide solution. A pH meter is used to monitor the pH so that there is a range from pH 1 to pH 11. To supply the enzyme catalase, equal pieces of liver is placed into each test tube. The rate of reaction is then measured by the height of oxygen bubbles produced.

Results: Catalase was most effective at a pH of 7. At pH 1 and pH 11 the reaction came to a stop.

Conclusion: The enzyme catalase is most effective at pH 7 and then the rate of reaction decreases as the pH increase or decreases from that point.