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3.9 perform a first-hand investigation to: extract the mixture of pigments from leaves examine the absorption spectrum of these pigments separate the pigments using chromatography

perform a first-hand investigation to:

  • extract the mixture of pigments from leaves
  • examine the absorption spectrum of these pigments
  • separate the pigments using chromatography

How you can measure the amount of pigment in plants (external website) Mad Science Network, Washington University, USA

Be aware that more sophisticated chromatography techniques than using solvents are now available to separate pigments in plants. Advanced spectrophotometers can precisely determine the absorption spectrum of plant pigments.

Extraction of pigments from spinach (external website) Classroom bats, Bermuda Biological Station for Research, Inc, Florida, USA